NHS Meningitides Real-TM,sacace -50t

Kit NHS Meningitidis Real-TM is a Real-Time test for the detection and differentiation of
Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumoniae in the biological
materials. DNA is extracted from specimens, amplified using RT-amplification and detected
using fluorescent reporter dye probes specific for N.meningitidis, H.influenzae, S.pneumoniae
DNA and IC (Internal Control).
MATERIALS PROVIDED
“Controls”
 Negative Control C- *, 1,2 ml;
 Internal Control (IC) **, 1,0 ml;
 Pos DNA N. meningitidis C+, 0,1 ml;
 Pos DNA H.influenzae C+, 0,1 ml
 Pos DNA S.pneumoniae C+, 0,1 ml;
 Pos IC C+, 0,1 ml;
 DNA-buffer, 0,5 ml;
“NHS Meningitidis Real-TM”: Real Time amplification
 PCR-mix-1 N. meningitidis/IC, 0,6 ml;
 PCR-mix-1 S. pneumoniae/ H. influenzae 0,6 ml;
 PCR-mix-2, 2 x 0,3 ml;
 TaqF Polymerase, 2 x 0,03 ml;
Contains reagents for 55 reactions
must be used in the isolation procedure as Negative Control of Extraction.
** add 10 µl of Internal Control during the DNA purification procedure directly to the sample/lysis mixture

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