Rotavirus/Norovirus/Astrovirus Real-TM,sacace-50t

Kit Rotavirus/Norovirus/Astrovirus Real-TM is a Real-Time test for the qualitative detection
and differentiation of Rotavirus A, Norovirus 2 genotype, Astrovirus in the biological materials
and in the environment. RNA is extracted from specimens, amplified using RT-amplification and
detected using fluorescent reporter dye probes specific for Rotavirus/Norovirus/Astrovirus RNA
and IC (Internal Control).
MATERIALS PROVIDED
“Rotavirus/Norovirus/Astrovirus Real-TM”: Real Time amplification kit
 PCR-mix-1 Rotavirus / Astrovirus, 0,6 ml;
 PCR-mix-1 Norovirus / IC 0,6 ml;
 RT-PCR-mix-2, 2 x 0,3 ml;
 Hot Start Taq Polymerase, 2 x 0,03 ml;
 M-MLV Revertase, 2 x 0,015 ml;
 RT-G-mix-2, 2 x 0,015 ml;
Contains reagents for 55 reactions
“Controls”
 Negative Control C-, 1,6 ml;*
 Internal Control (IC RNA), 5 x 0,12 ml.**
 Pos cDNA Rotavirus/Astrovirus C+, 0,1 ml;
 Pos cDNA Norovirus 2 / Internal Control (IC) C+, 0,1 ml;
 DNA-buffer, 0,5 ml;
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